Effect of a Mutation at Tyrosine 950 of the Insulin-like Growth Factor I Receptor on the Growth and Transformation of Cells1
نویسندگان
چکیده
Recent evidence has shown that the insulin-like growth factor I (IGF-I) receptor plays a major role in the establishment and maintenance of transformation. To identify domains in the IGF-I receptor that are nec essary for transformation, the tyrosine at residue 950 of the human IGF-I receptor cDNA was mutated to phenylalanine, and the plasmid expressing the mutant receptor was stably transfected into K cells, which are mouse embryo fibroblasts with a targeted disruption of the type 1 receptor for the insulin-like growth factors. At variance with the wild-type receptor, the Y950 mutant receptor has lost its ability to transmit an IGF-Imediated mitogenic signal or to transform K cells. These experiments show, for the first time, that tyrosine 950 of the IGF-I receptor is neces sary for its mitogenic and transforming activities.
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